Daily Archives: May 28, 2024

Effect of Unique Chromium Reductase Activity on Bioremediation of Chromium in Proteus SP. Isolated from Waste Water
Authors:-Assistant Professor Suhal Sardar, Assistant Professor Aabid Ahmad, Assistant Professor Anjali Jakhar, Assistant Professor Vikrant Kumar

Abstract-This is the first time where Proteus sp. has been isolated from waste water which has higher potential for bioremediation. Chromium (Cr) compounds are used in dyes and paints and in the tanning of leather. So they are found in soil and ground water at abundant industrial sites, now needing environmental clean-up and remediation. More toxic Cr (VI) is reduced by the chromium reducing bacteria to Cr(III) which is less toxic. Isolation of chromium reducing bacteria from water samples from the industrial area of muzaffarnagar was done to observe the effect of Chromium on them, to study their growth curve characteristics and for remediation assay of heavy metal contaminated industrial wastes by evaluating their Cr(VI) reducing ability through chromate reductase activity. Potassium dichromate (K2Cr2O7) and potassium chromate (K2CrO2) were employed for the growth of these bacteria. Attempts were made to isolate the genomic DNA of the organism and to amplify its 16S rRNA gene for identification of the organism using Bioinformatics tools. The organism identified was Proteus sp. Fourier transform infrared (FT-IR) spectroscopic study was performed to obtain information of the possible cell-metal ion interaction. This study has application in terms of bioremediation of metal contaminated industrial waste water with the bacteria isolated from waste water. This process is environment friendly and cost effective. Moreover the chromate reductase enzyme from isolated bacteria can be purified and immobilized which can be further used to detoxify of Cr from tannery wastes.

DOI: 10.61137/ijsret.vol.10.issue3.262

Published by: Kajal Tripathi

Isolation and Analysis of Amylase Producing Bacteria from Soil Samples
Authors:-Vipin Kumar Saini, Disha Sharma, Saba Rana, Zehra Husaini, Mohd Salman, Shalini Mishra, Shayma Saifi

Abstract-The aim of this study is to reveal the ability of various isolates obtained from soil to produce amylase enzyme. Soil samples were collected from Shri Ram College nursery. Amylase is an enzyme that catalyzes the hydrolysis of starch into simpler sugars such as maltose and glucose. It is produced by various organisms including bacteria, fungi, plants, and animals, and plays a crucial role in carbohydrate metabolism. A total of 10 species were isolated from soil. The two isolates were showed better results on Nutrient agar starch medium plates. A Gram stain test was carried out to identify the two isolates as Gram-positive rods. Morphological and biochemical analysis on the basis of standard indicated that they all associated mainly with members of the Bacillus sp.

DOI: 10.61137/ijsret.vol.10.issue3.261

Published by: Kajal Tripathi

Soil Microbial Communities in the Continuous Sugarcane Cropping Fields: A study
Authors:-Ashu Chaudhary, Vikas Kumar, Ankit Kumar, Zehra husaini, Vipin Kumar Saini, Shayma Saifi, Darshika Sharma, Vandana Sharma

Abstract-Soil microbial communities are pivotal for soil health and agricultural productivity, comprising bacteria, fungi, protozoa, and viruses that interact with each other and with plant roots. They break down organic matter, releasing essential nutrients, maintain soil structure, and help protect plants from disease and pests. However, human activities like land use change and chemical use can negatively impact these communities, leading to soil degradation and reduced crop productivity. Continuous sugarcane cropping exacerbates soil degradation, reducing fertility and increasing susceptibility to pests and diseases due to nutrient depletion, soil compaction, and altered microbial balance. To mitigate these impacts, practices such as crop rotation and organic matter application are crucial. This study examines the taxonomic and functional diversity of soil microbial communities in continuous sugarcane cropping fields in Uttar Pradesh. Methodologically, it identifies research questions, evaluates methodology, analyzes taxonomic and functional diversity, interprets results, and communicates findings effectively. Results indicate high microbial diversity but reduced abundance of beneficial taxa due to continuous sugarcane cropping, impacting soil health and sustainability. Adoption of sustainable agricultural practices is essential to support soil health and microbial diversity. Further research is needed to understand the long-term effects of continuous sugarcane cropping and develop mitigation strategies.

DOI: 10.61137/ijsret.vol.10.issue3.260

Published by: Kajal Tripathi