Isolation and Analysis of Amylase Producing Bacteria from Soil Samples
Authors:-Vipin Kumar Saini, Disha Sharma, Saba Rana, Zehra Husaini, Mohd Salman, Shalini Mishra, Shayma Saifi
Abstract-The aim of this study is to reveal the ability of various isolates obtained from soil to produce amylase enzyme. Soil samples were collected from Shri Ram College nursery. Amylase is an enzyme that catalyzes the hydrolysis of starch into simpler sugars such as maltose and glucose. It is produced by various organisms including bacteria, fungi, plants, and animals, and plays a crucial role in carbohydrate metabolism. A total of 10 species were isolated from soil. The two isolates were showed better results on Nutrient agar starch medium plates. A Gram stain test was carried out to identify the two isolates as Gram-positive rods. Morphological and biochemical analysis on the basis of standard indicated that they all associated mainly with members of the Bacillus sp.
