ISOLATION, PURIFICATION AND PARTIAL CHARACTERIZATION OF PROTEASE ENZYME FROM GUAVA (Psidium Guajava) LEAVES

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Authors: LOKESWARAN. V, SHANMUGAVADIVU. M

Abstract: Protease enzymes play an important role in many biological processes, including protein digestion, cell signals and protective mechanisms. In this study, the isolation, refining and partial characteristics of the protease enzyme of Guava (Psidium Guajava) have been studied. Dry guava leaves are homogeneous and extracted by raw enzymes using phosphate stamps. The activity of the protease has been determined by the digestion of casein and raw enzyme extract refined by precipitating in ammonium sulfate. The specific activity of pure protease enzyme is significantly higher than the rough extract. The enzyme is characterized by its pH, its optimal temperature and stability, showing the maximum operation at pH 4 and 60° C. In addition, the molecular weight of the protease enzyme is about 135 kda. In addition, the ability to decrease protein enzyme shows its ability to apply in soft meat, hydrolysed protein in food processing and remove points in laundry detergent. This study emphasized that the promising potential of guava panels is a profitable and environmentally friendly biological substance for industrial applications, especially in the fields of food and detergents. Add in -Depth on its complete enzyme records and its industrial scale is reasonable

DOI: http://doi.org/



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